Coding

Part:BBa_K2259019:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-01)


Beta-neomycin resistance protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 301
    Illegal SapI.rc site found at 511


Design Notes

Inserted a stop codon which was missing in Beta-neomycin.


Source

Sequence was acquired from supplementary material from Schmidt et al., 2012.

References